The intracytoplasmic sperm injection (ICSI) process involves the placement of single spermatozoon directly into the oooplasm of the denuded mature oocyte. The holding and injecting pipettes, which are to be fixed on the micromanipulator, are both made from borosilicate glass capillary tubes. A spike is present on the injected pipette for nontraumatic injection of the sperm into the oocyte. Both pipettes are bent, 1 mm from the tip at an angle of approximately 35 ◦C, to enable injection procedure to be performed with the tips positioned horizontally in an ICSI dish. The micromanipulator head stage is mounted on to the invert microscope with the joystick positioned remotely. A pair of motorized positioners, drop handle joystick micromanipulators and universal joints are combined as a set.
The incubator is a device that provides a constant temperature appropriate to cell culture. It also provides the sterile conditions for cultivation and the development of embryos. The Co2 Incubators deliver a higher level of performance for a dependable and reliable controlled in-vitro environment for optimum tissue cell culture growth by properly controlling temperature, humidity, CO2 gas, and sterility. The Co2 Incubators also provides an environment for the storage and preservation of embryos and cell cultures.
Phase Contrast Microscopy is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. One of the major advantages of phase contrast microscopy is that living cells can be examined in their natural state without previously being killed, fixed, and stained. As a result, the dynamics of ongoing biological processes can be observed and recorded in high contrast with sharp clarity of minute specimen detail. It reveals many cellular structures that are not visible. Phase Contrast Microscopy is suitable for sperm analyzing and morphology in ART and research laboratories.
The stereo, stereoscopic or dissecting microscope is an optical microscope variant designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it. The instrument uses two separate optical paths with two objectives and eyepieces to provide slightly different viewing angles to the left and right eyes. This arrangement produces a three-dimensional visualization of the sample being examined. Stereomicroscopy overlaps macrophotography for recording and examining solid samples with complex surface topography, where a three-dimensional view is needed for analyzing the detail.
The stereo microscope is often used to separation the oocyte from follicular fluid, cryopreservation and denudation in ART and research laboratories.
The laminar flow with HEPA filtration captures and removes airborne contaminants and provides a particulate – free work environment .Airflows is drawn around the operator into the font t grill of the cabinet, providing personnel protection and a downward flow of HEPA filtered air minimizes the chance of cross- contamination along the work surface. Class II Cabinets is self – contained with a front grill. An internal blower draws room air through a HEPA filter in the front grill. The laminar flow air splits as it approaches the work surface, so that approximately 70% of the airs is recirculated through the supply HEPPA filter back into the work zone, and 30% passes through the exhaust HEPA filter to the laboratory atmosphere and is recirculated. This is recommended for embryo culture procedures and medias.