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Equipment
A
gel doc
, also known as a gel documentation system, gel image system or gel imager, refers to equipment widely used in
molecular biology
laboratories for the imaging and documentation of nucleic acid and protein suspended within
polyacrylamide
or
agarose
gels.
[1]
These gels are typically stained with
ethidium bromide
or other nucleic acid stains such as
GelGreen
. Generally, a gel doc includes an
ultraviolet
(UV) light transilluminator, a hood or a darkroom to shield external light sources and protect the user from UV exposure, and a CMOS camera for image capturing.
The thermal cycler
(also known as a thermocycler, PCR machine or DNA amplifier) is a
laboratory
apparatus most commonly used to amplify segments of
DNA
via the
polymerase chain reaction
(PCR).
[1]
Thermal cyclers may also be used in laboratories to facilitate other temperature-sensitive reactions, including
restriction enzyme
digestion or rapid diagnostics.
[2]
The device has a thermal block with holes where tubes holding the reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps.
Date:
2019/09/18
Views:
195
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